Cordless-Microscope
All about Cordless-Microscope.ZSOLT SZABO MD, PhD
ZSOLT SZABO MD, PhD
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Other Articles:
| • | Immunolabeling for Correlative Light and Electron Microscopy on Ultrathin Cryosections |
| Research Articles Irawati K. Kandela, Reiner Bleher, Ralph M. Albrecht, Microscopy and Microanalysis, Volume 14 Issue 02 , pp 159-165 Abstract Correlative labeling permits colocalization of molecular species for observation of the same sample in light (LM) and electron microscopy (EM). Myosin bands in ultrathin cryosections were labeled using both fluorophore conjugated to secondary antibody (IgG) and colloidal gold (cAu) particles conjugated to primary IgG as reporters for LM and transmission electron microscopy (TEM), respectively. This technique allows rapid evaluation of labeling via LM, prior to more time-consuming observations with TEM and also yields two complementary data sets in one labeling procedure. Quenching of the fluorescent signal was inversely related to the distance between fluorophore and cAu particles. The signal from fluorophore conjugated to secondary antibody was inversely proportional to the size of cAu conjugated to primary antibody. Where fluorophore and cAu were bound to the same antibody, the fluorescence signal was nearly completely quenched regardless of fluorophore excitation or emission wavelength and regardless of particle size, 3 nm and larger. Colloidal metal particles conjugated to primary antibody provide high spatial resolution for EM applications. Fluorophore conjugated to secondary antibody provides spatial resolution well within that of conventional fluorescence microscopy. Use of fluorescent secondary antibody moved the fluorophore a sufficient distance from the cAu particles on the primary antibody to limit quenching of fluorescence. |
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| • | Tony Wright Asks If Brain Development Has Been Halted By By Eating Habits |
| A British plant biologist believes he has discovered why our brains stopped developing thousands of years ago and why we all have the potential to become geniuses. | |
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| • | A Phase I Study of Bolus 5-Fluorouracil and Leucovorin Combined with Weekly Paclitaxel (FLTAX) as First-line Therapy for Advanced Gastric Cancer |
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To determine the dose-limiting toxicity (DLT) and the maximum-tolerated dose (MTD) of combination chemotherapy with leucovorin-modulated weekly bolus 5-fluorouracil (5-FU) and weekly paclitaxel in patients with advanced gastric cancer (GC). Chemotherapy-naïve patients with histologically proven metastatic or recurrent GC were enrolled. Paclitaxel was administered as a 1-h intravenous (i.v.) infusion followed by 5-FU as a bolus i.v. infusion on Days 1, 8 and 15. A 2-h i.v. infusion of l-leucovorin was started at the same time as the paclitaxel infusion on Days 1, 8 and 15. Treatment cycles were repeated every 28 days until disease progression or unacceptable toxicity occurred. Patients were scheduled to receive 5-FU, l-leucovorin and paclitaxel at four dose levels (mg/m2/week): 500/250/60 (level 1), 500/250/80 (level 2), 600/250/80 (level 3) and 600/250/100 (level 4), respectively. Eighteen patients were enrolled. During the first cycle of the highest dose level (level 4), two of the six patients had DLT involving Grade 3 diarrhea and Grade 3 skin rash. Furthermore, three of the four patients who received the second consecutive cycle of treatment at dose level 4 had Grade 4 neutropenia. Dose level 3 was thus determined to be the MTD. Eleven (61%) of the 18 patients had partial responses, and the median progression-free survival time was 6.8 months. The MTD and the recommended dose for phase II studies of this regimen were determined to be 5-FU 600 mg/m2/week, l-leucovorin 250 mg/m2/week and paclitaxel 80 mg/m2/week. |
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| • | Congratulations to Dr Fiona Coyle |
| Congratulations to Dr Fiona Coyle of IEO, who today successfully defended her doctoral thesis entitled “Digital close range photogrammetry in motor vehicle accident reconstruction”. Examiners were (extern) Emeritus Professor Ray Brach, Department of Aerospace & Mechanical Engineering, University of Notre Dame, Notre Dame, Indiana, USA and (intern) Dr. Brian Bowe, Acting Assistant Head, School of Physics. Fiona’s research was supervised by Dr. Vincent Toal, Head of Physics, and Dr. Denis Wood, Wood and Associates, Forensic Engineers, Dublin. |
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| • | After-school Science for Adults? |
Apparently science after school is not just for kids. If you live in NYC, "Talking Science" offers events at a local bar that make science exciting and fun. The quote from the site that I think tells it all is: "[NPR Science Friday host] IRA FLATOW ON A BED OF NAILS!!" |
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| • | Heat- and electron-beam-induced transport of gold particles into silicon oxide and silicon studied by in situ high-resolution transmission electron microscopy |
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In this study, we describe the transport of gold (Au) nanoparticles from the surface into crystalline silicon (Si) covered by silicon oxide (SiO2) as revealed by in situ high-resolution transmission electron microscopy. Complete crystalline Au nanoparticles sink through the SiO2 layer into the Si substrate when high-dose electron irradiation is applied and temperature is raised above 150°C. Above temperatures of 250°C, the Au nanoparticles finally dissolve into fragments accompanied by crystallization of the amorphized Si substrate around these fragments. The transport process is explained by a wetting process followed by Stokes motion. Modelling this process yields boundaries for the interface energies involved. |
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