Cordless-Microscope
All about Cordless-Microscope.K-Wire Fixation for Extraarticular Transverse/Short Oblique Fractures of the Shaft of the Middle Phalanx Associated with Extensor Tendon Injury
K-Wire Fixation for Extraarticular Transverse/Short Oblique Fractures of the Shaft of the Middle Phalanx Associated with Extensor Tendon Injury
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In a prospective study, 22 cases of extraarticular transverse/short oblique fractures of the shaft of the middle phalanx associated with extensor tendon injury had fixation of the fracture as well as immobilisation of the distal interphalangeal joint using a K-wire. Mobilisation of the proximal interphalangeal and metacarpophalangeal joints was started immediately after surgery. The wires were removed after 6 weeks. No post-operative complications were noted. At final follow-up (mean = 15 weeks, range = 12–24 weeks), 18 of 22 patients obtained excellent and good total active motion (TAM) scores. Stiffness was confined to the distal interphalangeal joint, and hence when the results were re-analysed for motion at that joint only, only 11 patients had excellent and good outcomes.
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Other Articles:
| • | A Method for Chronological Intravital Imaging of Bovine Oocytes during In Vitro Maturation |
| Research Articles Morten R. Petersen, Michael Hansen, Birthe Avery, Ingrid B. Bøgh, Microscopy and Microanalysis, Volume 14 Issue 06 , pp 549-560 Abstract Oocyte maturation is known to affect the chances for successful fertilization, embryonic development, establishment of pregnancy and delivery of a live, healthy, and viable offspring. Two-photon laser scanning microscopy (TPLSM) has previously been used to evaluate early embryonic development without a detectable impairment of subsequent development, but has never been applied to assess mammalian oocytes throughout in vitro maturation (IVM). Visualization of structures within live oocytes during IVM, followed by fertilization and embryo culture, may improve the understanding of oocyte maturation. To visualize structures within bovine oocytes using TPLSM, it is necessary to remove the cumulus cells that normally surround the oocyte during maturation. Repeated visualization of structures within the same oocyte is possible, if movement of the oocyte can be avoided. In this article, we describe the development of a method for repeated intravital imaging of denuded bovine oocytes using an upright TPLSM equipped with a specially constructed incubator. Oocytes were stained with Hoechst 33258, and the nuclear structures were evaluated. Oocyte fertilization rate was not affected by TPLSM exposure, but the developmental capacity of the denuded oocytes was significantly reduced. This is, to our knowledge, the first article describing repeated intravital imaging during mammalian oocyte maturation using TPLSM. |
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| • | Association of Transforming Growth Factor-beta 1 Polymorphisms with Genetic Susceptibility to TNM Stage I or II Gastric Cancer |
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Transforming growth factor-beta 1 (TGF-β1) inhibits the proliferation of tumors in early stages of cancers, whereas it promotes tumor growth and metastasis in later stages of cancers. To examine the effect of the TGF-β1 polymorphisms on gastric cancer risk, we studied the association between C–509T and T+29C (Leu10Pro) polymorphisms in TGF-β1 and gastric cancer risk in 414 cases and 414 controls in the Chinese population. When the overall gastric cancer cases were compared with the controls, no significant difference was found in genotype distributions for both the polymorphisms examined. However, when stratified by tumor stage, the –509T and +29C allele carriers had a 0.57-fold (95% CI = 0.36–0.90) and a 0.58-fold (95% CI = 0.36–0.91) decreased risk of TNM stage I+II gastric cancer, respectively, as compared with non-carriers. We conclude that TGF-β1–509T and +29C alleles may have a protective role in the development of stage I+II gastric cancer. |
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| • | Congratulations to Dr Sharon McDermott |
| Our congratulations to Dr Sharon McDermott of the School of Physics who recently passed her PhD viva. Sharon worked on the use of UV-LEDs in the photopolymerisation of the biocompatable polymer HEMA. The external examiner was Prof Werner Blau from Trinity College Dublin and the DIT internal examiner was Dr Hugh Byrne. Sharon's supervisors were Dr Robert Howard and Dr James Walsh. |
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| • | Electron-microscopic observation of mouse spleen tissue infected with Orientia tsutsugamushi isolated from Shandong, China |
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Low-virulent Orientia tsutsugamushi (Ot) were successfully isolated from scrub typhus patients in Shandong, China, and the isolates were similar to the Kawasaki type identified by nested polymerase chain reaction (PCR). To identify the morphological characterization of the low-virulent Ot, and elucidate the pathological changes on host cells, mouse spleen tissue infected with the Ot isolated from Shandong was used for the ultrastructural study. Transmission electron microscopy showed that the Ot parasitized in the spleen were different in size, shape and electron density and many significant changes occurred in cytoplasmic organelles of the inoculated mouse spleen cells. Swollen perinuclear cisterna was observed in the nuclear membranes of mononuclear cells and a multivesicular body was found in the intracytoplasm of the macrophage. In the phagosome of the macrophage, many Ot enveloped with an additional membrane were found to push the phagosomal membrane outward from inside. The results indicated that the low-virulent Ot and the spleen cells suffered various damages. |
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