Cordless-Microscope
All about Cordless-Microscope.TimTec And Sigma-Aldrich Announce The Availability Of The MyriaScreen Diversity Collection
TimTec And Sigma-Aldrich Announce The Availability Of The MyriaScreen Diversity Collection
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Click Here to read the entire article...Other Articles:
| • | Seeing the Brain in Action: How Multiphoton Imaging Has Advanced Our Understanding of Neuronal Function |
| Research Articles Grace Stutzmann, Microscopy and Microanalysis, Volume 14 Issue 06 , pp 482-491 Abstract Gaining insight into how the nervous system functions is a challenge for scientists, particularly because the static morphology of the brain and the cells within tell little about how they actually work. Fixed specimens can provide critical structural information, but the jump to functional neurobiology in living cells is obviated with these preparations. In order to grasp the complexity of neuronal activity, it is necessary to observe the brain in action, from the level of subcellular signaling to the whole organism. Recent advances in nonlinear microscopy have given rise to a new era for biological research. In particular, the introduction of multiphoton excitation has drastically improved the depth and speed to which we can probe brain function. In order to better appreciate recent contributions of multiphoton microscopy to our current and future understanding of biological systems, an historical awareness of past microscopy applications is useful. |
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| • | Determination of Indication for Sentinel Lymph Node Biopsy in Clinical Node-negative Breast Cancer Using Preoperative 18F-fluorodeoxyglucose Positron Emission Tomography/Computed Tomography Fusion Imaging |
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Sentinel node biopsy (SNB) is indicated for axillary lymph node metastasis-negative cases (N0), but clarification of the indication may increase treatment efficiency. Fluorine-18-labeled 2-fluoro-2-deoxy- Ninety-two breasts/axillae were analyzed retrospectively in 90 patients (median age 54.6-year old, median primary tumor 1.7 cm). FDG-PET/computed tomography was used to indicate SNB in N0 cases. Axillary lymph node dissection (ALND) was performed in cases that were axillary lymph node metastasis-positive (PET N+) on FDG-PET/CT. Seventy-four (80.4%) and 18 (19.6%) of the 92 axillae were diagnosed as metastasis-negative (PET N0) and PET N+, respectively, by FDG-PET/CT. SNB was performed in 51 of the 74 PET N0 axillae. ALND was performed in 23 PET N0 axillae (at the patients’ request) and in all 18 PET N+ axillae. Of the 74 PET N0 axillae, 14 were metastasis-positive (pN+) and 60 were pN0 pathologically, and of the 18 PET N+ axillae, 13 were pN+ and five were pN0. The sensitivity and specificity of FDG-PET/CT for diagnosis of axillary metastasis were 48.1 and 92.3%, respectively, and the positive and negative predictive values were 72.2 and 81.1%, respectively. The positive detection rate on FDG-PET/CT was insufficient for determining an indication of SNB. However, use of an appropriate cut-off for SUVmax (the positive rate was 90.9% with a cut-off of 2.0) and exclusion of surgically biopsied cases may achieve a clinically applicable positive detection rate. |
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| • | Congratulations to Dr Pavani Kotakonda |
| Congratulations to Dr Pavani Kotakonda who successfully defended her doctoral thesis, entitled “Holographic Liquid Crystal devices”, on December 5th. The examiners were Prof. Gabriella Cipparrone of the Department of Physics at the University of Calabria, and Dr. John Doran, DIT. Pavani was supervised by Dr Robert Howard, Dr. Izabela Naydenova and Dr. Vincent Toal. |
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| • | Sports4Kids National Model |
Thanks to the Robert Wood Johnson Foundation, Sports4Kids, an Oakland, CA, based non-profit, is seeking to reach more than 1 million more children with its successful model of safe and healthy play. This is a great opportunity to expand a successful program, which teaches positive behaviors through sports and playground activities. The grant is for $18.7 million and seeks to reach 650 schools in 27 cities. For those interested in taking youth development to a larger scale audience, this is a huge step in the right direction. |
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| • | Correction of Swan Neck Deformity in Rheumatoid Arthritis Using a New Lateral Extensor Band Technique |
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Swan neck deformity is a progressive and disabling condition that commonly affects rheumatoid arthritic hands. During a 4-year period, 101 fingers in 43 patients had this deformity corrected using a new procedure combining the distally based extensor lateral band technique described by Littler and the flexor digitorum superficialis (FDS)-palmar plate pulley introduced by Zancolli. The ranges of motion of the metacarpophalangeal, proximal interphalangeal (PIP) and distal interphalangeal (DIP) joints were assessed pre-operatively and 12 months after surgery. An average PIP joint hyperextension of –13.3° was converted to +13.4°. The ranges of motion of the proximal and DIP joints were significantly different (Student’s t-test). No patient suffered recurrence of the deformity during an average follow-up of 20 months. This new technique improves some unappealing aspects of previous techniques and provides a stable and reliable correction of swan neck deformity. |
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| • | A high-throughput approach for cross-sectional transmission electron microscopy sample preparation of thin films |
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Cross-sectional transmission electron microscopy (XTEM) is a very useful technique to study the interfacial diffusion and reactions and the grain growth of thin films. However, the preparation of XTEM samples of thin films is tedious and challenging. Difficulties may include the delamination of films from the substrate, fracture of brittle substrates and differential milling rates of the substrate and the film. This paper describes an improved technique using a combination of tripod polishing and focused ion beam milling to prepare XTEM samples of thin films. The technique can be widely used for high-throughput production of samples having varying film and substrate properties. Two different geometries are introduced. The first one is suitable for XTEM sample preparation of most films at a high yield rate, but with a limited view area. The other geometry is able to give a larger view area and is more suitable for thicker films. The technique is illustrated by an example of the sample preparation of Fe/Pt multilayer films on SiO2/Si substrates. |
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